Spectroscopic Investigation of Increased Fluorescent Intensity of Fluorescent Dyes When Adsorbed onto Polystyrene Microparticles

Microplastics as environmental pollutants are increasingly a source of alarm. The characterization microplastics will be necessary to discriminate from other types particles. To specific microplastics, plastic-adsorbable fluorescent dyes used, the stained separated dye-microplastic mixture by filtration, and type staining is analyzed microscopy. In this study, realize in situ analysis staining, i.e., without any separation or filtration processes, we studied change properties after adsorption microplastic particle surfaces using 3D excitation emission matrix fluorescence spectroscopy (the wavelength-dependent spectrum). We used three dyes: Fluorescein, Rhodamine 6G, Methylene Blue, polystyrene microparticles our model microplastic. Fluorescein Blue showed increases intensity, while 6G negligible intensity changes. This likely due degree affinity surface, structural stability on changes environment around each dye surface. conclude that have demonstrated potential look for appropriate method here identify estimate individual plastic materials.